Mouse microglial cell lines differing in constitutive and interferon-γ-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells
William S. Walker, Janet Gatewood, Elvia Olivas, David Askew, Carin E. G. Havenith
Journal of Neuroimmunology Volume 63, Issue 2, 31 December 1995, Pages 163-174
We developed a panel of non-virus transformed cell lines derived from individual microglial precursors residing in the brains of normal mice.
These colony stimulating factor-1-dependent cell lines are B7-1 + (CD80), Mac-1 +, Mac-2+, Mac-3+, CD45+, MHC class I+, colony stimulating factor-1 receptor+, and they ingest antibody-coated particles.
However, the cell lines differ in their expression of B7-2 (CD86), F4/80, Ly-6C and MHC class II molecules. They also differ in their ability to constitutively process and present antigens to naive CD4+ and CD8+ T cells, memory CD4+ and CD8+, and in the manner by which interferon γ modulates their antigen-presenting activities.
These cell lines should be valuable as models for studies on the immunobiology of the microglia.